Multi View MuVi-SPIM

Life in the Fast Lane

Highest Imaging Speed on the Market
Gentle Sample Handling and Extremely Low Phototoxicity
Multiview Imaging without Rotation
High Sensitivity and Low Noise
Ideal for Imaging Dynamic Process in Living Specimen

The MuVi-SPIM utilizes a sheet of laser light to illuminate only a thin slice of a fluorescently labeled sample. A wide-field fluorescence microscope, placed perpendicular to the light-sheet, serves to collect the fluorescence signal and images the observed region by means of a camera. The MuVi-SPIM provides four simultaneous orthogonal views on large living specimens without the need for sample rotation. This avoids shadowing eﬀects and facilitates long-term imaging at dramatically increased acquisition speed. Modular software concepts allow the flexible design of complex experimental layouts. 3D image data are used in real-time and directly streamed to a storage and data processing server.

MuVi-SPIM system uses some unique and some patented technologies. The four objective lenses allow four combinations of illumination and detection. This provides four orthogonal views of the specimen without the need for sample rotation. It enables multiple view imaging of large samples at unprecedented speed with long-term stability. MuVi-SPIM is the fastest SPIM on the market for analyzing dynamic processes, which allows 4 simultaneous views at 65 frames/sec. Extremely low phototoxicity and photobleaching, and high imaging speed make MuVi-SPIM an ideal system for imaging dynamic processes in live specimen, as well as applications such as:

Developmental Biology
Embryonic Development
Marine Biology
Neurobiology/Neuro-development
Functional Imaging
Long Time-lapse Imaging
Large Specimen

A fruit fly embryo from when it was about two-and-a-half hours old until it walked away from the microscope as a larva, acquired by a MuVi-SPIM system. Credit to: Mette Handberg from Pavel Tomancak’s lab, MPI-CBG, Dresden, Germany

3D construction images of zebrafish and zebrafish eye, acquired by a MuVi-SPIM system. Credit to: Andrew Oates, Francis Crick Institute, London, UK

Imaging of Zebrafish heart. Credit to: Inês Marques & Nadia Mercader, Uni Bern, Switzerland

A Dye (Bengal rose) was injected into the Duct of Cuvier of a 2d old zebrafish larvae. Thereby all vessels were stained. Credit to: A. Machate and M. Brand, CRTD, TU Dresden

Spheroids from MCF-7 cells stained for actin (green), nuclei (blue, DNA dye Picogreen) and CK818 (red).. Credit to: Anna Taubenberger, Biotec TU Dresden

Image of Platynereis Dumerilii.The staining is EMTB, which is a microtubule associated protein, so what you see are microtubules. Credit to: Yu-Wen Hsieh & Pavel Tomancak, MPI-CBG, Dresden, Germany

Laser	Laser combiner with six laser positions
	405, 445, 488, 515, 532, 561, 594, 642, and 685nm @ 40mW
	Fast modulation and high extinction
Illumination optics	Two identical illumination arms
	Chromatic correction from 440 to 660 nm
	Light-sheet generation by beam scanning
	Variable light-sheet thickness (optional)
	Water-dipping objective lenses (see below)
	Easy and intuitive alignment
Detection Optics	Two identical detection arms
	Water-dipping objective lenses (see below)
	Fast filter wheels with 10 positions and 50 ms between adjacent positions
	High-speed sCMOS cameras Hamamatsu Orca Flash 4.0 v3 with 2048 x 2048 pixels of 6.5 µm x 6.5 µm size
Objective lens mounting unit	High precision mounting unit
	Fast exchange of the objective combination unit with high precision
	Mounting chamber with temperature control (see below)
	2 x Nikon CFI Plan Fluor 10X @0.3 NA water immersion for illumination
	2 x Olympus 20X @ 1.0 NA water immersion for detection
	Other objective lenses on request
Sample chamber and stage	Water-sealed inert PEEK plastic chamber, autoclavable and biocompatible
	Sample supported from below for improved stability
	Easy access from above for sample mounting, injections, etc.
	Fast and precise temperature control, range 15-40°C
	High-precision XYZ and fast rotation stage
	XYZ piezo crawler stage with 100 nm resolution and 9.5 mm (X) and 1.2 mm (YZ) travel range for fast sample positioning and stack acquisition
	Rotation stage with > 360 degrees/s speed @ 1 degree resolution
Electronics, Computer, Software	All electronic components included in the microscope housing
	Embedded microscope software with open communication interface
	Open GUI control for interactive control and microscope automation
	Computer with 128 GB RAM, Intel Dual Six Core CPU
	High-speed RAID controller and 10 Gb/s Ethernet port for data streaming
	8 x 4 TB local storage in RAID 0
	One 40 inch monitor

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Objectives

Objectives Close View

Objectives

System Overview

Sideview

Close View

System Overview