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SECOM

Ultrastructure in the Spotlight

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SECOM is  a unique  integrated  solution  for correlative light and  electron microscopy that enables extremely  fast  correlative  microscopy with  the highest  optical  quality  and  overlay  accuracy.  SECOM is the only product to date that  integrates fluorescence  and  scanning  electron microscopy  in  one device by equipping a scanning  electron  microscope (SEM)  with  an   inverted  fluorescence microscope  (FM).  With  its  integrated  design,  switching  from  fluorescence  to   electron  imaging  is  seamless  and  instantaneous.    SECOM is  installed as  a retrofit to  an  existing SEM  by  replacing  the  vacuum  chamber  door.  SECOM  is  fully   compatible  with  all  the standard  detectors, allowing using the full potential of existing electron microscope. 

 

Schematic overview SECOM system 

 

 

SECOM system is being used for applications including cancer research, marine biology, neuro-science and cell biology. SECOM SR, an integrated CLEM system with a super-resolution optical microscope is also available.  

Courtesy of T. Templier and R.H.R.Hahnloser, University of Zurich and ETH Zurich

Courtesy of  C.J.Peddie and L.M.Collinson, The Francis Crick Institute, U.K

Side of SECOM

 

 

Fluorescence  Multiband setup in Pinkel configuration optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores.
Default excitation wavelengths at 387/11, 485/20, 560/25 and 650/13nm. Others available on request.
Camera  sCMOS, 2048 x 2048 pixels with a pixel size of 6.5 um yields.
330x330um field of view at 40x magnification.
Sample Stage  Piezo-electronic stepper motor and optical linear encoders.
Travel range of 18x18mm in XY and step size of 300nm.
Lens system   Plan Apochromat Objective Lens 40x/0.95 (dry)
Other objective lens, including oil immersion lenses (up to NA 1.45), available on request.
User exchangeable.
CE certified Yes
RoHS compliant Yes

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Recent news
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