Ultrastructure in the Spotlight
SECOM is a unique integrated solution for correlative light and electron microscopy that enables extremely fast correlative microscopy with the highest optical quality and overlay accuracy. SECOM is the only product to date that integrates fluorescence and scanning electron microscopy in one device by equipping a scanning electron microscope (SEM) with an inverted fluorescence microscope (FM). With its integrated design, switching from fluorescence to electron imaging is seamless and instantaneous. SECOM is installed as a retrofit to an existing SEM by replacing the vacuum chamber door. SECOM is fully compatible with all the standard detectors, allowing using the full potential of existing electron microscope.
Schematic overview SECOM system
SECOM system is being used for applications including cancer research, marine biology, neuro-science and cell biology. SECOM SR, an integrated CLEM system with a super-resolution optical microscope is also available.
Courtesy of T. Templier and R.H.R.Hahnloser, University of Zurich and ETH Zurich |
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Courtesy of C.J.Peddie and L.M.Collinson, The Francis Crick Institute, U.K
Side of SECOM
| Fluorescence | Multiband setup in Pinkel configuration optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores. |
| Default excitation wavelengths at 387/11, 485/20, 560/25 and 650/13nm. Others available on request. | |
| Camera | sCMOS, 2048 x 2048 pixels with a pixel size of 6.5 um yields. |
| 330x330um field of view at 40x magnification. | |
| Sample Stage | Piezo-electronic stepper motor and optical linear encoders. |
| Travel range of 18x18mm in XY and step size of 300nm. | |
| Lens system | Plan Apochromat Objective Lens 40x/0.95 (dry) |
| Other objective lens, including oil immersion lenses (up to NA 1.45), available on request. | |
| User exchangeable. | |
| CE certified | Yes |
| RoHS compliant | Yes |
SECOM Overview
HeLa cells(C.J. Peddie,CRUK LRI)
Seamless integration with SEM
Pancreas (Ben Giepmans)