Double Dutch: Bright Resolution
RCM2 is the second generation re-scan confocal microscope from Confocal.nl with digital scanner technology. It makes bi-directional scanning the standard and allows an acquisition speed of 2fps at 512×512 pixels.
RCM2 is suitable for super-resolution imaging with high NA objectives in the low magnification range, like 40x 1.4. A lower magnification allows for a bigger field of view (FOV), brighter images, and even lower laser power. RCM2 has been demonstrated to be able to perform excellent imaging at 10 nano-watt excitation power.
Capture datasets of 170nm resolution raw (120 after deconvolution) over a very large FOV. Study fast live-cell dynamics and perform 4D imaging in optimized conditions. The increased detection efficiency facilitates acquisition in low fluorescence conditions, like single-molecule detection (smFISH).
In combination with silicon objectives, RCM2 also allows for deep 3D imaging of organoids, zebrafish embryos, or larger live samples.
RCM Image in Normal Res and High Res
From left to right: Neutrons & Neuroblastom
From left to right: Mitotic Cell (original) & Mitotic Cell (De-convoluted)
|Lasers||Support up to 4 laser lines: from 405nm upwards|
|Additional laser wavelengths upon request|
|3rd party laser combiner supported|
|Laser output through single line fiber (FC Connector)|
|Microscope||Standard microscope with C-mount adapter|
|Major brand microscopes supported|
|Camera||Hamamatsu ORCA-Flash 4.0 (V2 or V3)|
|Andor Zyla, iXON 888, 897 & Ikon|
|PCO Edge 4.2|
|Photometrics Prime 95B|
|Other major EMCCD/sCMOS camera supported (min 6.45um pixel size)|
|Features||170nm lateral resolution (FWHM @ 488nm), 120nm with deconvolution. 500nm axial resolution.|
|220×220 micron field of view (40x, super-resolution)|
|2 fps acquisition speed at 512×512 pixels|
|50um pinhole size (2.2AU for 100x objective)|
|Up to 95% Quantum Efficiency|
|Software||RCM drivers available for LabVIEW, Micromanager, and Fiji/ImageJ|
|Support for NIS Elements, Zen, LAS X, Cellsens, Volocity|