About

RCM2 is the second generation re-scan confocal microscope from Confocal.nl with digital scanner technology. It makes bi-directional scanning the standard and allows an acquisition speed of 2fps at 512×512 pixels.

RCM2 is suitable for super-resolution imaging with high NA objectives in the low magnification range, like 40x 1.4. A lower magnification allows for a bigger field of view (FOV), brighter images, and even lower laser power. RCM2 has been demonstrated to be able to perform excellent imaging at 10 nano-watt excitation power. 

Potential Applications

Capture datasets of 170nm resolution raw (120 after deconvolution) over a very large FOV. Study fast live-cell dynamics and perform 4D imaging in optimized conditions. The increased detection efficiency facilitates acquisition in low fluorescence conditions, like single-molecule detection (smFISH).

In combination with silicon objectives, RCM2 also allows for deep 3D imaging of organoids, zebrafish embryos, or larger live samples.

Neurons in co-culture stained for Actin (red), MAP2 (magenta) and Tau (green). Imaged on RCM2 with a 40x 1.4 objective. Sample courtesy: Vera Wiersma, VU University, Amsterdam, the Netherlands.

Re-scan Confocal Microscopy (RCM) is a super-resolution technique based on standard laser-scanning confocal technology. With a second scanner of the emission  fluorescence, the image results in resolutions up to 170nm. With the high sensitivity of the camera-based detection and the open pinhole design,  the RCM requires very low laser power and offer a very high signal-to-noise ratio, while maintaining confocal sectioning capability.  The re-scanning principle is optics-mechanics only, achieving an improved resolution image without any post-processing. Therefore the RCM can be added smoothly to your existing fluorescence microscope.

RCM Image in Normal Res and High Res  

RCM  is an  affordable, high resolution and sensitive confocal imaging system – an ideal solution for labs with limited budget, but demanding tasks,  especially when high sensitivity and  high resolution are desired from the imaging system. 

Possible applications of RCM including: • Super-resolution imaging • Living Cells • Ratio-imaging • Multi-color applications 

 

From left to right: Neutrons & Neuroblastom

  From left to right: Mitotic Cell (original) & Mitotic Cell (De-convoluted)