The LCS SPIM is a compact light-sheet microscope designed for fast and gentle 3D imaging of large, optically-cleared samples. The LCS SPIM’s compact, robust and vibration-free design provides maximal stability even during long-term high-throughput experiments. The following webinar includes a demonstration of imaging using the LCS SPIM.
In the latest version of Arivis Vision4D, users will be able to run customised python scripts in the pipeline itself. In this demonstration, chromatin in the nucleus is precisely segmented into concentric rings and quantified using a python script. This implementation further improves the flexibility and power of the Arivis Vision4D pipeline, which has already…
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Researchers from the Centre for Organismal Studies in Heidelberg, Germany have recently published their research in the journal eLife. The Imaging Machine from Acquifer was used to image live medaka and zebrafish samples over 20 hours at a 30 min interval. Optimal temperatures of 26°C for medaka and 28°C for zebrafish were maintained throughout imaging.…
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To investigate the formation of breast cancer tumours, researchers from EMBL (Heidelberg, Germany) used the Invi SPIM from Luxendo to perform long-term live cell imaging (up to 4 days). This was possible due to the low phototoxicity and high resolution provided by the Invi SPIM system. The authors reported that only small local clusters of…
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StarDist is a deep learning-based method for the segmentation of nuclei in 2D and 3D. Compared to traditional image segmentation, StarDist performs much better in the segmentation of small and roundish objects, especially in situations where the objects are very close together or overlapping. Arivis AG has recently published a detailed protocol on performing StarDist…
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A recent publication in Nature highlighted the important role of actin and mitochondrial comet tails in symmetric cell division. Spinning disk images of mitochondria and actin (LifeAct) were deconvolved with Huygens software. Moore, A.S., Coscia, S.M., Simpson, C.L. et al. Actin cables and comet tails organize mitochondrial networks in mitosis. Nature 591, 659–664 (2021). Abstract…
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To investigate chromatin localization in the nucleus, researchers at the Weizmann Institute of Science used the scripting capability of Arivis Vision4D to implement a customized python script into their image-processing workflow. They managed to split the nucleus into multiple concentric radial shells in three dimensions (see Figure 1A), allowing them to precisely measure the fluorescence…
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The MuVi SPIM was used in a Science publication in July 2020 (Vol 369, Issue 6500). The article is titled “Contact area–dependent cell communication and the morphological invariance of ascidian embryogenesis” by Lea Guignard Abstract Marine invertebrate ascidians display embryonic reproducibility: Their early embryonic cell lineages are considered invariant and are conserved between distantly related…
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The first Re-Scan Confocal(RCM) from Confocal.nl was installed and successfully handed over at the prestigious National Centre for Biological Centre (TIFR-NCBS) Bangaluru. The RCM was installed on a Nikon Inverted Ti2E Microscope at Prof. Vardharajan Sudaramurthy’s Lab. The researchers are excited to see great improvement in image-resolution with the ease of controlling RCM through NIS elements software and high resolution…
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It has been a difficult time for everyone in the past few months due to CoVID-19. We sincerely hope everyone stays safe and well. We have been forced to stay at home but this also gives us a good opportunity to finally process the large amount of demo dataset acquired in various demonstrations and workshops.…
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