Another RCM has been installed in University of Wurzburg, Markus Sauer lab. PhD student Andreas Kurz will be the main user of the system.
Below are some of the result image and
video from this system:
COS7 cells, standard staining (actin – Alexa488, tubulin – Alexa647, and nucleus – DAPI)
Time lapse with RCM: HeLa cells expressing EB1 tagged with YFP. 2 seconds time interval, (original time lapse 40 minutes), acquired with 8µW laser power (by Andreas Kurz).
The specification for the existing microscope is:
Nikon TiE; Zyla 4.2P, Cobolt Skyra multiline laser, and imaging software NIS Elements with RCM plugin.
The setup will be used as a fast, resolution enhanced and life cell capable system together with a super-resolution technique (dSTORM). Applications include complementary imaging of receptors, cell migration assays, cytoskeleton dynamics and photo-toxicity studies.
In the future, RCM will also be used for neuronal studies, to take advantage of the drastically reduced excitation power.
The main reason for choosing the RCM was the overall package of technical specifications, possibility to integrate RCM to an existing system, and outstanding fair price. Andreas’s favorite features of RCM are Multi-color capability and the incredibly low laser power, which should prove useful for live cell experiments.
